Biotinylated Molecular Weight Marker
SIGMA/B2787 - mol wt 6,500-180,000 Da
Synonym: protein markers; protein molecular weight markers; protein standards
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 6,500-180,000 Da |
| packaging | vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose) |
| Quality Level | 200  |
| storage temp. | −20°C |
| usage | 1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels) |
| Application: | For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438 ) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures. |
| Other Notes: | Contains nine biotinylated proteins (approx 0.1 mg total). |
| Other Notes: | This product contains: • α2-macroglobulin (equine serum) • β-galactosidase (E. coli) • phosphorylase b (rabbit muscle) • catalase (bovine liver) • alcohol dehydrogenase (yeast) • carbonic anhydrase (bovine erythrocyte) • trypsin inhibitor (soy bean) • lysozyme (chicken egg) • aprotinin (bovine lung) Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose. |
| Preparation Note: | Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |