Bluo-Gal

SIGMA/B2904 - reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-3-indolyl β-D-galactopyranoside; substrate for β-galactosidase; Bluo-Gal

CAS Number: 97753-82-7
Empirical Formula (Hill Notation): C₁₄H₁₆BrNO₆
Molecular Weight: 374.18
MDL Number: MFCD00063691
Linear Formula: C₁₄H₁₆BrNO₆
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B2904-100MG	100 mg	$178.00 1/EA	Add To Favorites
45-B2904-500MG	500 mg	$694.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B2904-100MG

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
form	powder
grade	Molecular Biology
InChI	1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChI key	LINMATFDVHBYOS-MBJXGIAVSA-N
Quality Level	200
SMILES string	OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
solubility	DMF: soluble
sterility	non-sterile
storage temp.	−20°C
suitability	suitable for substrate for β-galactosidase
technique(s)	nucleic acid detection: suitable

Application:	Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Application:	Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
Biochem/physiol Actions:	When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
General description:	Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Packaging:	100, 500 mg in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352200

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