Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal
SIGMA/B3148 - clone GT-34, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Goat/Sheep IgG; Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse
Product Type: Chemical
ELISA Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in Mouse, Clone: GT-34 (Cat. No. B3148), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Goat/Sheep IgG by indirect ELISA. Reactivity is observed with Goat IgG and Sheep IgG but not with Bovine IgG and Human IgG.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GT-34, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human |
| species reactivity | sheep, goat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:5,000 |
| Application: | Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in: • enzyme linked immuno sorbent assay (ELISA) • immunoblotting • dot blotting • immunohistochemistry • immunocytochemistry |
| Application: | Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2. |
| General description: | The product binds to all goat/sheep IgG1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |