Monoclonal Anti-Bcl-2 antibody produced in mouse
SIGMA/B3170 - clone Bcl-2-100, ascites fluid
Synonym: Anti-BCL-2
MDL Number: MFCD00239644
Product Type: Chemical
Western Blotting JOURNAL CITATION: Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer. By: Wang, Y., Kuramitsu, Y., et al. in PLoS One, 2014. PubMed ID: 25271986 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0109076), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting HeLa cell (human) extract was separated on SDS-PAGE and probed with Monoclonal AB to BCL-2 (MO) CL:BCL-2-100 (Cat. No. B3170). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and an NBTBCIP substrate. Antibody dilution 1/2,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Bcl-2-100, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 26 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | mouse |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using human HeLa cell extract | |
| UniProt accession no. | P10415  |
| Application: | Anti-BCL2 (Ab-56) antibody has been used in western blotting and immunocytochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Bcl-2 antibody produced in mouse is suitable for western blotting at a concentration of 1:1,000 by using human HeLa cell extract. It is also suitable for immunohistochemistry by using formalin-fixed, paraffin-embedded tissue sections or frozen sections. Further, the antibody may be used for the localization of Bcl-2, by implementing numerous assays like- microarray, electron microscopy and immunoprecipitation. |
| Biochem/physiol Actions: | BCL2 (B-cell lymphoma 2) plays an important role in the growth and progression of cancer. Overexpression of BCL2 (B-cell lymphoma 2) results in poor clinical predictions in diffuse large B-cell lymphoma (DLBCL). Bcl2 is highly essential for the survival of kidney and melanocyte stem cells and mature lymphocytes. It may control Bcl2 multiple initiator caspases. |
| Biochem/physiol Actions: | Protooncogene BCL2 encodes a 26 kD integral outer mitochondrial membrane protein expressed in sub-cellular components such as the mitochondria and nucleus. While mitochondrial BCL2 inhibits apoptosis, BCL2 present in the nucleus may activate cell death. It may also regulate autophagy during starvation. Additionally, BCL2 facilitates the survival of central and peripheral neurons grown in culture in the absence of neurotrophic factors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | BCL2 (B-cell lymphoma 2) consists of pro- and antiapoptotic functions. It belongs to BCL2 family of proteins. It is mapped to human chromosome 18q21.3. |
| Immunogen: | synthetic peptide corresponding to residues 41-54 of the Bcl-2 protein, conjugated to thyroglobulin. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |