Bromphenol Blue–Xylene Cyanole Dye solution
SIGMA/B3269 - lyophilized powder
Product Type: Chemical
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| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark blue |
| form | lyophilized powder |
| pH range | 8-8.6 |
| Quality Level | 200  |
| solubility | water: 0.2 vial/mL, blue |
| storage temp. | room temp |
| Application: | Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel. |
| General description: | Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing. |
| Preparation Note: | Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360FD |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 60-61-36/37/38 |
| Safety Statements | 53-26-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |