Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse
SIGMA/B3275 - clone RG-16, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:3,000 Monoclonal Anti-Rabbit Immunoglobulins-Biotin antibody produced in Mouse, Clone: RG-16 (Cat. No. B3275) and 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Monoclonal Anti-Rabbit Immunoglobulins-Biotin antibody produced in Mouse, Clone: RG-16 (Cat. No. B3275) and 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:200,000 antibody 2. 1:400,000 antibody 3. Negative Control
ELISA Monoclonal Anti-Rabbit Immunoglobulins-Biotin antibody produced in Mouse, Clone: RG-16 (Cat. No. B3275), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) was probed to detect Rabbit Immunoglobulins by indirect ELISA. Reactivity is observed with Rabbit IgG, Rabbit IgA and Rabbit IgM but not with Human IgG, Human IgA and Human IgM.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-16, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human |
| pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep | |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:60,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500 | |
| western blot: 1:200,000 | |
| western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well) |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Application: | The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature. |
| Biochem/physiol Actions: | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |