Anti-Human IgG2−Biotin antibody, Mouse monoclonal
SIGMA/B3398 - clone HP-6014, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG2
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG2- Biotin antibody produced in mouse, Clone HP-6014 (Cat. No. B3398), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Human IgG2 by indirect ELISA. Reactivity is observed with Human IgG2k and Human IgG2 λ but not with Human IgG1 λ, Human IgG3 λ and Human IgG4 λ.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6014, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| Application: | Monoclonal Anti-Human IgG2−Biotin antibody produced in mouse has been used in indirect enzyme linked immunosorbent assay (ELISA) and immunoblot. |
| Biochem/physiol Actions: | IgG provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Low IgG2 levels were found in patients with systemic lupus erythematosus (SLE) and juvenile diabetes melitus. |
| Biochem/physiol Actions: | The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG2 (mouse IgG1 isotype) is derived from the hybridoma (HP-60141 ) produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG is the most abundant serum immunoglobulins of the immune system. IgG2 is a subtype of IgG. IgG is secreted by B cells and is found in blood and extracellular fluids. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |