Anti-Human IgG4−Biotin antibody, Mouse monoclonal
SIGMA/B3648 - clone HP-6025, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG4
Product Type: Chemical
ELISA Anti-Human IgG4-Biotin antibody, Mouse monoclonal, Clone: HP-6025 (Cat. No. B3648), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Human IgG4 by direct ELISA. Reactivity is observed only with Human IgG4 but not with HuIgG1, HuIgG2 and HuIgG3.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6025, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:60,000 |
| Application: | Anti-Human IgG4−Biotin antibody, Mouse monoclonal has been used in immunohistochemistry and immunostaining. |
| Application: | Monoclonal Anti-Human IgG4-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:15,000. It was used for detection of serum IgG4 produced in response to malaria parasite infection by ELISA. |
| Application: | The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG4 antibody allows for the binding of various labels such as avidin or streptavidin. |
| Biochem/physiol Actions: | IgG4 is the least abundant immunoglobulin present in the human serum. IgG4 is implicated in a unique process called fab-arm exchange (FAE) and limits the formation of immune complex. It has anti-inflammatory properties. IgG4 functions as a therapeutic antibody. It provides protection against allergy, prevents mast cell degranulation and has a harmful role in malignant melanoma. IgG4 may be associated with autoimmune diseases such as, autoimmune pancreatitis (AIP) type 1 and 2, Mikulicz syndrome, Kuttner′s tumor, Riedel′s thyroiditis and tubulointerstitial nephritis. |
| Biochem/physiol Actions: | Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |