Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal
SIGMA/B3773 - clone HP-6017, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG (Fc specific)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:20 Anti-Human IgG (Fc specific)-Biotin antibody, Mouse monoclonal, Clone: HP-6017 (Cat. No. B3773) followed by 1:800 Monoclonal Anti-Biotin-Cy3™ antibody produced in Mouse, Clone: BN-34 (Cat. No. C5585).
ELISA Anti-Human IgG (Fc specific)-Biotin antibody, Mouse monoclonal, Clone: HP-6017 (Cat. No. B3773), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Human IgG by indirect ELISA. Reactivity is observed only with Human IgG but not with HuFab, HuIgM, HuIgA and Kappa+Lambda.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6017, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, sheep, horse (IgG), goat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:80,000 |
| Application: | Anti-Human IgG (Fc specific) Biotin antibody, Mouse monoclonal has been used in fluoroimmunoassay of plasma samples. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) |
| Application: | Monoclonal Anti-Human IgG (Fc specific)-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:80,000. It was used for labeling mouse splenocytes and analysis by flow cytometry. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin. |
| Biochem/physiol Actions: | IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG generates fragment crystallisable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies. |
| Biochem/physiol Actions: | The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Immunoglobulin super-family have a common structure, comprising of two heavy (H) chains and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |