BCIP®/NBT-Blue Liquid Substrate System for Membranes
SIGMA/B3804 - alkaline phosphatase substrate
Synonym: 5-
Product Type: Chemical
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.
| form | liquid |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis. |
| Disclaimer: | Stable at least one year at 2-8 °C. This substrate is light-sensitive. |
| General description: | Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency. |
| Legal Information: | BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Not recommended for microwell (e.g., ELISA) or immunohistochemical applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |