BIS-TRIS

SIGMA/B4429 - BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol; 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

CAS Number: 6976-37-0
Empirical Formula (Hill Notation): C₈H₁₉NO₅
Molecular Weight: 209.24
EC Number: 230-237-7
MDL Number: MFCD00002853
Linear Formula: C₈H₁₉NO₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B4429-25G	25 g	$73.90 1/EA	Add To Favorites
45-B4429-100G	100 g	$195.00 1/EA	Add To Favorites
45-B4429-500G	500 g	$827.00 1/EA	Add To Favorites
45-B4429-1KG	1 kg	$1390.00 1/EA	Add To Favorites

ATR-IR Spectra for 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol.

FT-IR Spectra for 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B4429-100G

Properties
Descriptions
Safety Info.
Basic Data

anion traces	≤5 ppm
application(s)	diagnostic assay manufacturing
assay	≥98.0%
form	crystalline powder
grade	BioPerformance Certified
impurities	endotoxin and total aerobic microbial count, tested
InChI	1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
InChI key	OWMVSZAMULFTJU-UHFFFAOYSA-N
mp	104 °C
pKa (25 °C)	6.5
Quality Level	400
SMILES string	OCCN(CCO)C(CO)(CO)CO
solubility	H₂O: 500 mg/mL, clear, colorless to very faintly yellow
suitability	suitable for supravital staining of living blood cells
technique(s)	cell culture \| insect: suitable
	cell culture \| mammalian: suitable
useful pH range	5.8-7.2

Application:	A new test for the laboratory diagnosis of spherocytosis: This study introduces a novel diagnostic assay utilizing BIS-TRIS buffer, aimed at improving the detection and characterization of spherocytosis in clinical settings. The buffer′s stability and optimal pH make it suitable for routine use in clinical laboratories, contributing to accurate and reproducible diagnostic results (Vettore et al., 1984 ).
General description:	Bis-Tris is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The useful pH range of Bis-Tris is 5.8 - 7.2.
Other Notes:	Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table .
Packaging:	1 kg in poly bottle
Packaging:	100, 500 g in poly bottle

Symbol	GHS05
Signal word	Danger
Hazard statements	H318
Precautionary statements	P280 - P305 + P351 + P338 + P310
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98.0%
mp	104 °C
UNSPSC	12161700

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