Anti-53BP1 (C-terminal) antibody produced in rabbit
SIGMA/B4436 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-TP53BP1; Anti-p53 Binding Protein 1
MDL Number: MFCD09039188
Product Type: Chemical
Immunoprecipitation Lanes 1, 2, 3. 5, 2, 5 and 1.0 μg of Rabbit Anti-53BP1 (C-ter), Cat. No. B4436 was used to immunoprecipitate human 53BP1 from 0.25 mg of 293T cell lysates; the immunoprecipitation was detected with antibody. rabbit anti 53BP1 (N-ter), Cat. No. B4561, and developed with Goat anti-Rabbit IgG, Peroxidase conjugate followed by a chemiluminescent substrate. 4. Negative control — without cell lysate 5. Negative control — without IP antibody 6. Positive control — Extract of 293-T cells was separated on SDS-PAGE and blotted with Rabbit Anti-53BP1 (N-ter) (Cat. No. 4561)
Western Blotting U2OS total cell extracts were separated on SDS-PAGE and probed with Anti-53BP1 (C-terminal) antibody produced in Rabbit (Cat. No. B4436) followed by 1:30,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 4 μg/mL antibody 2. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 345 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse (predicted), rat (predicted), human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK 293-T cell lysates |
| microarray: suitable | |
| western blot: 2-4 μg/mL using U2OS total cell extracts | |
| UniProt accession no. | Q12888  |
| Application: | Anti-53BP1 (C-terminal) antibody produced in rabbit has been used in western blotting and immunoprecipitation |
| Biochem/physiol Actions: | Tumor protein p53 binding protein (T153BP1) has a close link to ataxia-telangiectasia mutated (ATM)-regulated events. 53BP1 becomes phosphorylated by ATM in a DNA damage-dependent manner suggesting that it participates in propagating the ATM signaling to its downstream effectors. T153BP1 also plays a key role in DNA repair by mediating nonhomologous end-joining (NHEJ) near double stranded breaks (DSBs). Fusion of T153BP1 with platelet-derived growth factor receptor beta (PDGFRβ) is implicated in a type of myeloproliferative disorder accompanied with eosinophilia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tumor protein p53 binding protein (T153BP1) is mapped to human chromosome 15q15.3. It has methylated histone-binding module and tandem tudor domains. |
| Immunogen: | synthetic peptide corresponding to amino acids 1955-1972 of human 53BP1, conjugated to KLH via an N-terminal added cysteine residue. This sequence is conserved in mouse, and differs from rat by one amino acid |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |