Bromelain from pineapple stem
SIGMA/B4882 - ≥3 units/mg protein
CAS Number: 37189-34-7
EC Number: 253-387-5
MDL Number: MFCD00081423
Product Type: Chemical
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| composition | Protein, ≥30% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥3 units/mg protein |
| storage temp. | −20°C |
| Application: | Bromelain from pineapple stem has been used: • to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants • as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide ge • to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences |
| Application: | Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen . |
| Biochem/physiol Actions: | Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanil |
| Other Notes: | One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C). |
| Preparation Note: | The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.32 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |