Capillary Electrophoresis Running Buffer (10x)
SIGMA/B4930 - for automated DNA sequencing
Synonym: Running Buffer; Sequencing Buffer
Product Type: Chemical
Data quality analysis based on PHRED q>20 scores. Average read length of both single-stranded and double-stranded DNA samples were analyzed on the ABI Prism® 3700 under standard run conditions of 3 hours at 1,000V, 50 °C. Samples were prepared using BigDye™ terminators with SigmaSpin™ post-reaction clean up.
Sequencing reaction was purified using a SigmaSpin 96-well plate. pFLAG™ MAC plasmid (Sigma, E 5644) was sequenced using BigDye™ Terminator v 3.0 chemistry (20 μl total reaction volume; BigDye premix diluted 1:1 with SeqSaver™ [Sigma, S 3938]). Data was generated on an ABI Prism® 3700 DNA Analyzer with POP-6™ polymer and 10X CE Buffer (Sigma, B 4930). Phred 20 > 600.
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | DNA sequencing: suitable |
| type | for automated DNA sequencing |
| Application: | Capillary Electrophoresis Running Buffer (10x) has been used for automated sequencing by capillary electrophoresis. |
| Features and Benefits: | • Compatible with ABI Prism 3700 DNA Analyzer and Molecular Dynamics MegaBACE capillary electrophoresis instruments • Provides consistency and high performance for every batch • Stable at room temperature for convenient shipping, and storage • Tested and works well with the performance-optimized polymer 6 (POP6) • Cost-efficient - high-quality buffer at a great value • Quality control testing of every lot ensures proper pH, conductivity, and performance for Capillary Electrophoresis Sequencing • Plastic bottles provide easy handling • Available in one, four and 20 L as well as custom packaging options available |
| Other Notes: | Capillary Electrophoresis Buffer is for laboratory use only. Not for drug, household, or other uses. |
| Preparation Note: | Product should be diluted to 1x before use. Add 1 volume of the 10X capillary electrophoresis buffer solution to 9 volumes of deionized water. Cover the top of the container and mix the 1X solution by inversion. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H290 - H314 |
| Precautionary statements | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| Hazard Codes | C |
| Risk Statements | 35 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 1824 8 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |