5-Bromo-2′-deoxyuridine

SIGMA/B5002 - ≥99% (HPLC)

Synonym: 5-BrdU; 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil; 5-Bromouracil deoxyriboside; BUdR

CAS Number: 59-14-3
Empirical Formula (Hill Notation): C₉H₁₁BrN₂O₅
Molecular Weight: 307.10
EC Number: 200-415-9
MDL Number: MFCD00006529
Linear Formula: C₉H₁₁BrN₂O₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B5002-100MG	100 mg	$65.60 1/EA	Add To Favorites
45-B5002-250MG	250 mg	$82.90 1/EA	Add To Favorites
45-B5002-500MG	500 mg	$134.00 1/EA	Add To Favorites
45-B5002-1G	1 g	$164.00 1/EA	Add To Favorites
45-B5002-5G	5 g	$616.00 1/EA	Add To Favorites
45-B5002-100G	100 g	$8820.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B5002-100G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (HPLC)
biological source	synthetic (organic)
form	powder
InChI	1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key	WOVKYSAHUYNSMH-RRKCRQDMSA-N
mp	191-194 °C (dec.) (lit.)
Quality Level	200
SMILES string	OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
solubility	DMSO: 50 mg/mL, clear, colorless to very faintly yellow
storage temp.	−20°C

Application:	5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
Application:	5-Bromo-2′-deoxyuridine has been used: • as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis • as a supplement to study the loss-of-gene function in adult zebrafish heart • in T lymphocyte proliferation assay{133]
Biochem/physiol Actions:	5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.
Biochem/physiol Actions:	Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
Packaging:	1, 5, 100 g in glass bottle
Packaging:	100, 250, 500 mg in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H340 - H361fd
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	46-61
Safety Statements	53-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Purity	≥99% (HPLC)
mp	191-194 °C (dec.) (lit.)
Storage Temp.	−20°C
UNSPSC	41106305

To Order From a Quote