Anti-Bak antibody produced in rabbit
SIGMA/B5897 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Bcl-2 Homologous Antagonist/Killer
MDL Number: MFCD00282578
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human colon carcinoma sections with 1:100 Rabbit Anti-Bak (Cat. No. B5897) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Whole extract of A431 cells were separated on SDS-PAGE and probed with Anti-Bak antibody produced in Rabbit (Cat. No. B5897). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. 1:8,000 antibody 5. 1:2,000 antibody + inhibition with 5 μg/mL immunizing peptide 6. 1:2,000 antibody + inhibition with 10 μg/mL immunizing peptide 7. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 28 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using sections of human colon carcinoma |
| microarray: suitable | |
| western blot: 1:2,000 using human epidermal carcinoma A431 whole cell extract | |
| UniProt accession no. | Q16611  |
| Application: | Anti-Bak antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Bak (Bcl-2 homologous antagonist/killer, Bak1) is involved in regulating apoptosis. Bak can accelerate the rate of apoptosis when overexpressed in some cell lines. Increased Bak expression in normal and neoplastic intestinal epithelial cells results in apoptosis. However, expression of Bak in a human lymphoblastoid cell line, provided protection from apoptosis induced by serum deprivation and the oxidant menadione, suggesting that the function of Bak may be context dependent. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Bak (Bcl-2 homologous antagonist/killer, Bak1) belongs to the B-cell lymphoma 2 (Bcl-2) family of proteins. The bak gene is mapped to chromosome 6 and encodes a 233 amino acid protein with a predicted MW of 23.4 kDa. Bak shares homology with Bcl-2 in the Bcl‐2 homology (BH) domains (BH1 and BH2). Bak is expressed in a wide variety of cell types and tissues, with the highest levels observed in heart and skeletal muscle. |
| Immunogen: | synthetic peptide corresponding to the N-terminus of human Bak amino acids 23-38 with C-terminally added lysine, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |