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X-GlcA

SIGMA/B6650 - reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; X-GlcA; X-glucuronide CHA salt

CAS Number: 114162-64-0
Empirical Formula (Hill Notation): C14H13BrClNO7 · C6H13N
Molecular Weight: 521.79
MDL Number: MFCD00058543
Linear Formula: C14H13BrClNO7 · C6H13N
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B6650-10MG 10 mg
 $174.00
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45-B6650-25MG 25 mg
 $287.00
1/EA		 Add To Favorites
45-B6650-100MG 100 mg
 $863.00
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assay ≥98% (TLC)
form powder
grade Molecular Biology
InChI 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
InChI key JXCKZXHCJOVIAV-CYRSAHDMSA-N
Quality Level 200 
SMILES string NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
solubility DMF: soluble
sterility non-sterile
storage temp. −20°C
suitability suitable for β-galactosidase test
technique(s) nucleic acid detection: suitable
Application: Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Application: Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
Biochem/physiol Actions: When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
General description: X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (TLC)
Storage Temp. −20°C
UNSPSC 12352200

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