Advanced Search



X-GlcA

SIGMA/B6650 - reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; X-GlcA; X-glucuronide CHA salt

CAS Number: 114162-64-0
Empirical Formula (Hill Notation): C14H13BrClNO7 · C6H13N
Molecular Weight: 521.79
MDL Number: MFCD00058543
Linear Formula: C14H13BrClNO7 · C6H13N
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B6650-10MG 10 mg
 $187.00
1/EA		 Add To Favorites
45-B6650-25MG 25 mg
 $308.00
1/EA		 Add To Favorites
45-B6650-100MG 100 mg
 $924.00
1/EA		 Add To Favorites

 

assay ≥98% (TLC)
form powder
grade Molecular Biology
InChI 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
InChI key JXCKZXHCJOVIAV-CYRSAHDMSA-N
Quality Level 200 
SMILES string NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
solubility DMF: soluble
sterility non-sterile
storage temp. −20°C
suitability suitable for β-galactosidase test
technique(s) nucleic acid detection: suitable
Application: Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Application: Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
Biochem/physiol Actions: When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
General description: X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (TLC)
Storage Temp. −20°C
UNSPSC 12352200

The following items have been added to your cart:

Choose a favorite list for this item:
Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top