Anti-Human IgG1−Biotin antibody, Mouse monoclonal
SIGMA/B6775 - clone 8c/6-39, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG1 (Fc specific); Anti Human IgG1 - Monoclonal Anti-Human IgG1-Biotin antibody produced in mouse; Anti Human Igg1
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG1 -Biotin antibody produced in Mouse, Clone 8c/6-39 (Cat. No. B6775), followed by 2 μg/mL ExtrAvidin® -Peroxidase (Cat. No. E2886), was serially diluted to detect Human IgG1 by indirect ELISA. Reactivity is observed with Human IgG1 κ but not with Human IgG2 κ, Human IgG3 κ and Human IgG4 κ.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | 8c/6-39, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:1,000 |
| Application: | Monoclonal Anti-Human IgG1-Biotin antibody is suitable for use in immunoblot. The product can also be used for direct ELISA (1:1,000). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders Monoclonal Anti-Human IgG1-Biotin antibody is specific for an epitope in the Fc region of the human IgG1. The product does not react with human IgG2, IgG3, or IgG4. This antibody clone (HP6019 clone) has been recognized as a useful human IgG1 specificity standard in the WHO/IUIS study . |
| General description: | The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study. |
| Immunogen: | Fc fragment of myeloma Human IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |