Bovine Serum Albumin
SIGMA/B6917 - lyophilized powder, suitable for (for molecular biology), Non-acetylated, Molecular Biology
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
| assay | ≥98% (agarose gel electrophoresis) |
| biological source | bovine |
| foreign activity | Alkaline phosphatase and peroxidase, none detected |
| Alkaline Phosphatase free | |
| DNase free | |
| DNase, RNase, and protease, none detected | |
| Peroxidase free | |
| Protease free | |
| RNase free | |
| form | lyophilized powder |
| grade | Molecular Biology |
| impurities | enzymatic impurity, none detected |
| mol wt | ~66 kDa |
| origin | USA origin |
| packaging | glass bottle of |
| pH | 7 |
| purified by | heat shock fractionation |
| quality | deacetylated |
| Quality Level | 300  |
| solubility | water: soluble (50 mg/ml) |
| storage temp. | 2-8°C |
| suitability | suitable for (for molecular biology) |
| technique(s) | electrophoresis: suitable-98% using Agarose |
| UniProt accession no. | P02769  |
| Application: | Bovine Serum Albumin (BSA) has been used • as a component of phosphate buffer saline (PBS) solution for dissolving tumor necrosis factor α (TNFα), which was added as a supplement for culture media • as a blocking agent to reduce non-specific binding during the albumin sensor assay • as a blocking agent in the immunofluorescence (IF) assay • as a protein standard in the Bradford assay |
| Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is a versatile protein commonly utilized in cell and tissue culture media as a supplement. It helps in lipid solubilization and stabilizing other proteins. BSA is also a carrier for small molecules such as steroids, fatty acids, and thyroid hormones. It serves as a standard protein for immunochemical tests to determine protein concentration in a solution and calibration studies. |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Features and Benefits: | This BSA product for molecular biology is tested for the following properties: * Alkaline Phosphatase-free * DNase-free / Exonuclease-free * Nickase-free / Endonuclease-free * Peroxidase-free * Protease-free * RNase-free |
| General description: | Bovine Serum Albumin (BSA) is a plasma protein produced in the bovine liver that has a globular, non-glycosylated α-helical structure. It consists of a single polypeptide chain with 583 amino acid residues and 17 cysteine residues that form the cross-linked disulfide bridges, stabilizing its structure. The BSA protein comprises three structurally distinct domains (I, II, and III), each of which contains two sub-domains (A and B) arranged in a heart-shaped architecture. |
| Packaging: | 25, 100 mg in glass bottle |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (agarose gel electrophoresis) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |