Anti-Rat IgG (whole molecule)–Biotin antibody produced in goat
SIGMA/B7139 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162810
Product Type: Chemical
Frozen sections of mouse spleen stained for (M & m) B cells using Goat Anti-Mouse IgM Biotin conjugate (Cat. No. B9265) and for (N & n) L3T4 (helper/inducer T cells) using Goat Anti-Rat IgG Biotin conjugate (Product No. B 7139). Visualized using ExtrAvidin-peroxidase (Cat. No. E2886), DAB and nickel chloride, then counterstained with methyl green. "m" (100X) of the rectangular area in "M" (40X) shows germinal center formation. "n" (100X) of the rectangular area in "N" (40X) shows involvement of helper T cells in the germinal center formation. From W. Lee, Finch Univ. of Health Science, Chicago Med. School, Dept. Microbiology/Immunology, N. Chicago, IL.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:20,000 |
| Application: | Anti-Rat IgG (whole molecule)-Biotin antibody is suitable for use in immunohistochemistry and sandwich ELISA. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins Anti-Rat IgG (whole molecule)-Biotin antibody binds to all rat immunoglobulins. |
| Immunogen: | Purified rat IgG |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |