Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
SIGMA/B7401 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG
MDL Number: MFCD00162627
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded Human appendix tissue sections stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:400 Anti-Mouse IgG (Fc specific)-Biotin antibody produced in Goat (Cat. No. B7401) and by 25 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:160,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 | |
| western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well) |
| Application: | Anti-Mouse IgG (Fc specific)−Biotin antibody produced in goat has been used in ELISA and quantitative suspension array technology (qSAT) multiplex antibody assay. |
| Application: | Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin may be used to detect mouse IgG within serum or other biological systems. The antibody may be used as a secondary antibody in immunochemical assays based on biotin:avidin second antibody signal systems wherein mouse IgG is the primary antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin reacts with mouse IgG and mouse IgG, Fc fragment. It shows no reactivity with mouse IgG Fab fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG. |
| General description: | Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule. |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse or human samples. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |