Anti-c-Myc−Biotin antibody, Mouse monoclonal
SIGMA/B7554 - clone 9E10, purified from hybridoma cell culture
Synonym: Anti-c-Myc; Monoclonal Anti-c-Myc−Biotin antibody produced in mouse
Product Type: Chemical
Immunoblotting
Extract of 293-T cells transfected with pCDNA 3.1 (-)/Myc-His/LacZ plasmid (Cat. No. C3215) (Lanes 1, 2) and extract of 293-T untransfected (Lane 3) were separated on SDS-PAGE and blotted with Monoclonal Anti-c-Myc-Biotin (Cat. No. B7554). The antibody was developed with ExtrAvidin®-Peroxidase (Cat. No. E2886) and chemiluminescent substrate. (Lanes 1, 3) Antibody concentration 0.05 mg/mL; (Lane 2) Without primary antibody + ExtrAvidin -Peroxidase dilution: 1:20,000.
Extract of 293-T cells transfected with pCDNA 3.1 (-)/Myc-His/LacZ plasmid (Cat. No. C3215) (Lanes 1, 2) and extract of 293-T untransfected (Lane 3) were separated on SDS-PAGE and blotted with Monoclonal Anti-c-Myc-Biotin (Cat. No. B7554). The antibody was developed with ExtrAvidin®-Peroxidase (Cat. No. E2886) and chemiluminescent substrate. (Lanes 1, 3) Antibody concentration 0.05 mg/mL; (Lane 2) Without primary antibody + ExtrAvidin -Peroxidase dilution: 1:20,000.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9E10, monoclonal |
| concentration | 0.8 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein |
| UniProt accession no. | P01106  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) |
| Application: | The antibody is suitable for immunoblotting. |
| Biochem/physiol Actions: | Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435). |
| General description: | The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein. |
| Immunogen: | synthetic peptide of the human p62c-Myc protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |