5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

SIGMA/B8503 - ≥99% (HPLC)

Synonym: 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt; BCIP^® p-toluidine salt; X-phosphate p-toluidine salt

CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C₈H₆BrClNO₄P · C₇H₉N
Molecular Weight: 433.62
EC Number: 229-506-1
MDL Number: MFCD00040636
Linear Formula: C₈H₆BrClNO₄P · C₇H₉N
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B8503-25MG	25 mg	$73.30 1/EA	Add To Favorites
45-B8503-50MG	50 mg	$84.20 1/EA	Add To Favorites
45-B8503-100MG	100 mg	$120.00 1/EA	Add To Favorites
45-B8503-500MG	500 mg	$356.00 1/EA	Add To Favorites
45-B8503-1G	1 g	$574.00 1/EA	Add To Favorites
45-B8503-5G	5 g	$2330.00 1/EA	Add To Favorites

Thoracic and abdominal segments of a Drosophila melanogaster embryo double labeled using monoclonal antibodies to engrailed (purple) and achaete (brown) proteins. Engrailed expression marks the posterior compartment of each segment and achaete expression labels five neuroblasts per hemisegment: three found midway between engrailed stripes and two found coincident with engrailed stripes. The cellular outlines of non-achaete positive neuroblasts are clearly visible interspersed among the achaete positive neuroblasts. Goat Anti-Mouse IgG-Alkaline Phosphatase conjugate (Cat. No. A7434) and BCIP/NBT (Cat. Nos. B8503/N6876) and DAB tablets (Cat. No. D5905) were used. From J. Skeath, Univ. of Wisc., Madison, WI]

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (HPLC)
form	powder
InChI	1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key	QEIFSLUFHRCVQL-UHFFFAOYSA-N
Quality Level	200
SMILES string	Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
solubility	DMF: 20 mg/mL, clear to slightly hazy, yellow
storage temp.	−20°C

Application:	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.
Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules.
Biochem/physiol Actions:	5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.
General description:	5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.
General description:	Histochemical substrate for alkaline phosphatase.
Legal Information:	BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352204

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