Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit
SIGMA/B8520 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD00162641
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Musclet (Cat. No. A2547) followed by 1:300 Anti-Mouse IgG (whole molecule)-Biotin antibody produced in rabbit (Cat. No. B8520) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B8520) followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:800 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:200,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300 | |
| western blot: 1:400,000-1:800,000 |
| Application: | Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in • immunofluorescence • immunoblot • direct enzyme linked immunosorbent assay (ELISA) • serotyping assay • immunohistochemistry |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. |
| Immunogen: | Purified mouse IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |