Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
SIGMA/B8895 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162783
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human tonsil stained with Anti-Human IgG (whole molecule) antibody produced in rabbit (Cat. No. I8635) followed by 1:800 Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in goat (Cat. No. B8895) and by ExtrAvidin®-FITC (Cat. No. E2761).
Western Blotting Whole extract of Hela cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:400,000 antibody 2. 1:800,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:200,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 | |
| western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well) |
| Application: | Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in • Immunofluorescence • Immunohistochemistry • Western blot • Direct enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG. |
| Biochem/physiol Actions: | Binds all rabbit Igs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG. |
| Immunogen: | Purified rabbit IgG |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |