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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

SIGMA/B9146 - ≥98%

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG; X-Gal

CAS Number: 7240-90-6
Empirical Formula (Hill Notation): C14H15BrClNO6
Molecular Weight: 408.63
EC Number: 230-640-8
MDL Number: MFCD00005666
Linear Formula: C14H15BrClNO6
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B9146-10MG 10 mg
 $55.80
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45-B9146-100MG 100 mg
 $217.00
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45-B9146-500MG 500 mg
 $857.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B9146-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B9146-500MG

 

assay ≥98%
form powder
InChI 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key OPIFSICVWOWJMJ-AEOCFKNESA-N
Quality Level 200 
SMILES string OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
solubility DMF: methanol (1:1): soluble 50 mg/mL
storage temp. −20°C
suitability suitable for identification of lac+ bacterial colonies
Application: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
Application: X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Packaging: 10, 100, 500 mg in poly bottle
Preparation Note: A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98%
Storage Temp. −20°C
UNSPSC 12352204

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