Anti-HA-Biotin antibody, Mouse monoclonal
SIGMA/B9183 - clone HA-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-HA; Monoclonal Anti-HA-Biotin antibody produced in mouse; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Western Blotting Whole extract of E. coli cells expressing HA tag were separated on SDS-PAGE. The antibody was probed and developed with Monoclonal Anti-HA-Biotin antibody produced in Mouse, Clone: HA-7 (Cat. No. B9183) followed by ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| 2-8°C | |
| technique(s) | western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. |
| Application: | Antibody suitable for immunoblotting. |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| General description: | The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C; 2-8°C |
| UNSPSC | 12352203 |