Anti-Mouse IgM (μ-chain specific)–Biotin antibody produced in goat
SIGMA/B9265 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162658
Product Type: Chemical
Frozen sections of mouse spleen stained for (M & m) B cells using Goat Anti-Mouse IgM Biotin conjugate (Cat. No. B9265) and for (N & n) L3T4 (helper/inducer T cells) using Goat Anti-Rat IgG Biotin conjugate (Product No. B 7139). Visualized using ExtrAvidin-peroxidase (Cat. No. E2886), DAB and nickel chloride, then counterstained with methyl green. "m" (100X) of the rectangular area in "M" (40X) shows germinal center formation. "n" (100X) of the rectangular area in "N" (40X) shows involvement of helper T cells in the germinal center formation. From W. Lee, Finch Univ. of Health Science, Chicago Med. School, Dept. Microbiology/Immunology, N. Chicago, IL.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:20,000 |
| Application: | Anti-Mouse IgM (μ-chain specific)-Biotin antibody is suitable for use in immunocytochemistry (at 1:500 dilutions in methanol-fixed, sheep embryo sections) and direct ELISA (1:20,000). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . Anti-Mouse IgM (μ-chain specific)-Biotin antibody is specific for mouse IgM when tested against mouse IgA, IgG, and IgM by ELISA. |
| Immunogen: | Mouse IgM |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |