Anti-Avidin antibody, Mouse monoclonal, biotin conjugate
SIGMA/B9655 - clone WC19.10, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Avidin
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rabbit spleen tissue sections were stained with 100 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) followed by 1:100 ExtrAvidin®-FITC (Cat. No. E2761) and 50 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886) and by 1:2,000 Anti-Avidin antibody, Mouse monoclonal, biotin conjugate, Clone: WC19.10 (Cat. No. B9655)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | WC19.10, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: suitable |
| dot blot: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| Application: | Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women |
| Biochem/physiol Actions: | Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin. |
| Immunogen: | Purified egg white avidin. |
| Physical form: | Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |