BIS-TRIS

SIGMA/B9754 - ≥98.0% (titration)

Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol; 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

CAS Number: 6976-37-0
Empirical Formula (Hill Notation): C₈H₁₉NO₅
Molecular Weight: 209.24
EC Number: 230-237-7
MDL Number: MFCD00002853
Linear Formula: C₈H₁₉NO₅
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B9754-25G	25 g	$67.40 1/EA	Add To Favorites
45-B9754-100G	100 g	$163.00 1/EA	Add To Favorites
45-B9754-500G	500 g	$590.00 1/EA	Add To Favorites
45-B9754-1KG	1 kg	$1020.00 1/EA	Add To Favorites
45-B9754-5KG	5 kg	$3940.00 1/EA	Add To Favorites

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This image depicts SKU: B9754-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing general analytical microbiology
assay	≥98.0% (titration)
color	white
form	crystalline powder
InChI	1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
InChI key	OWMVSZAMULFTJU-UHFFFAOYSA-N
pKa (25 °C)	6.5
Quality Level	400
SMILES string	OCCN(CCO)C(CO)(CO)CO
solubility	H₂O: 500 mg/mL, clear, colorless to very faintly yellow
storage condition	dry at room temperature
suitability	suitable for column chromatography of proteins
	suitable for Western blot
technique(s)	electrophoresis: suitable
	UV/Vis spectroscopy: suitable
useful pH range	5.8-7.2

Application:	Bis-Tris has been used : • as a buffer for Electrophoresis • as a component of transfer buffer for Western Blotting
Features and Benefits:	• Suitable for Biological and Biochemical Research • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)
General description:	Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	100, 500 g in poly bottle
Packaging:	5 kg in poly drum

Symbol	GHS05
Signal word	Danger
Hazard statements	H318
Precautionary statements	P280 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Purity	≥98.0% (titration)
UNSPSC	12161700

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