Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
SIGMA/B9904 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162627
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:300 Anti-Mouse IgG (Fc specific)-Biotin antibody produced in Goat (Cat. No. B9904) and by 25 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
ELISA Anti-Mouse IgG (Fc specific)-Biotin antibody produced in Goat (Cat. No. B9904), followed by 1 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Mouse IgG by direct ELISA. Reactivity is observed with Mouse IgG and Mouse Fc but not with: Mouse Fab, Rat IgG, Human IgG, Human IgA and Human IgM.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | ≥2 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300 | |
| western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells |
| Application: | Anti-Mouse IgG (Fc specific)-Biotin antibody has been used in enzyme linked immunosorbent assay (ELISA), dot blot, immunohistology, nuclear labeling, ELISA-inhibition method. immunohistochemistry. |
| Application: | Anti-Mouse IgG (Fc specific)-Biotin antibody produced in goat was used for immunocytochemistry staining to visualize neonatal myosin in chicken muscle sections. It was used for ELISPOT analysis to study IgG levels in mice infected with cholera toxin. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bon |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |