Cathepsin B from human placenta
SIGMA/C0150 - lyophilized powder, ≥5 units/mg protein
Synonym: Cathepsin B1
CAS Number: 9047-22-7
MDL Number: MFCD00130748
Product Type: Chemical
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| composition | Protein, ~50% Lowry |
| form | lyophilized powder |
| mol wt | 24.5 kDa |
| Quality Level | 200  |
| solubility | H2O: soluble 1.0 mg/mL, clear |
| specific activity | ≥5 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P07858  |
| Application: | Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG). It has also been used in determining the effect of cysteine protease inhibitors. |
| Application: | Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer’s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells. |
| Biochem/physiol Actions: | Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced. |
| Biochem/physiol Actions: | Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer . |
| General description: | Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1. Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover. |
| Other Notes: | One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C. |
| Packaging: | 2, 10 units in serum bottle |
| Physical form: | Lyophilized powder containing phosphate buffer salts |
| Preparation Note: | Dissolves in water at 1 mg/mL concentration, yieliding a clear solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |