Monoclonal Anti-CD11b antibody produced in mouse
SIGMA/C0551 - clone ICRF44, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-CD11b
Product Type: Chemical
Flow Cytometry 1×106 Human lymphocytes were labeled with 1 μg/test Anti-CD11b antibody, Mouse monoclonal, Clone: ICRF44 (Cat. No. C0551) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Yellow histogram: antibody specific staining.
Flow Cytometry 1×106 Human monocytes were labeled with 1 μg/test Anti-CD11b antibody, Mouse monoclonal, Clone: ICRF44 (Cat. No. C0551) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Yellow histogram: antibody specific staining.
Flow Cytometry 1×106 Human granulocytes were labeled with 1 μg/test Anti-CD11b antibody, Mouse monoclonal, Clone: ICRF44 (Cat. No. C0551) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Yellow histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ICRF44, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 165-170 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5 μL using 1 × 106 cells |
| UniProt accession no. | P11215  |
| Application: | Monoclonal Anti-CD11b antibody produced in mouse has been used in immunostaining and flow cytometry. |
| Biochem/physiol Actions: | CD11b/CD18 acts as a receptor for C3bi, clotting factor X, fibrinogen and ICAM-1. It is involved in cell-cell and cell-substrate interactions. It plays a crucial role in several immunological processes as well including leukocyte extravasation and phagocytosis. It also plays an important role in certain coagulation protease cascade and in cell migration. |
| Biochem/physiol Actions: | Recognizes the CD11b α-chain of the human CD11b/CD18 complex. The antibody is useful for studies on cell adhesion and migration and for the detection of leukocyte adhesion deficiencies. It may be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive. 2nd Workshop: code no. M104 3rd Workshop: code no. 202 and 238 4th Workshop: code no. M47 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD11b α-chain of the human CD11b/CD18 complex is an α/β heterodimeric glycoprotein which belongs to the β2 integrin family. It is also known as Mac-1, CR3, MO-1 and C3bi receptor. CD11b is expressed on the surface of circulating monocytes, granulocytes and certain NK cells and is also present in subsets of macrophages. It functions as a receptor for iC3b, clotting factor X, fibrinogen and ICAM-1. |
| General description: | CD11b is also known as Mac-1, CR3, MO-1 and C3bi receptor. It belongs to β 2 integrin family. The gene encodes the integrin α M chain. It is expressed on the surface of circulating monocytes, granulocytes, neutrophils, macrophages, dendritic cells and certain NK cells. It is present in subcellular granules in granulocytes and on subsets of macrophages. |
| General description: | Monoclonal anti-Human CD11b antibody (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human monocytes. |
| Immunogen: | human monocytes. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |