Calcein
SIGMA/C0875 - Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
Synonym: Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein; Fluorescein-
CAS Number: 154071-48-4
Empirical Formula (Hill Notation): C30H26N2O13
Molecular Weight: 622.53
MDL Number: MFCD00005049
Linear Formula: C30H26N2O13
Product Type: Chemical
ATR-IR Spectra for Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein.
FT-IR Spectra for Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0875-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0875-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0875-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0875-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0875-5G
| application(s) | diagnostic assay manufacturing hematology histology |
| color | yellow to brown |
| description | mixed isomers |
| fluorescence | λex 470 nm; λem 509 nm (pH 7.4) |
| form | powder |
| InChI key | DEGAKNSWVGKMLS-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | OC(=O)CN(CC(O)=O)Cc1cc2c( |
| solubility | NaOH: 50 mg/mL |
| storage temp. | room temp |
| suitability | suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg) |
| technique(s) | titration: suitable |
| Application: | Calcein has been used: • in whole cell staining • in histomorphometry and micro-computed tomography • to label active bone-forming surfaces |
| Biochem/physiol Actions: | Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacet |
| Packaging: | 5, 10, 25 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |