Anti-Catalase antibody ,Mouse monoclonal
SIGMA/C0979 - clone CAT-505, purified from hybridoma cell culture
Synonym: Anti-CAT
MDL Number: MFCD00802760
Product Type: Chemical
Immunoblotting Human HepG2 cell extract was separated on SDS-PAGE and probed with Monoclonal PA to Catalase CL:CAT-505 (PREPK) (Cat. No. C0979). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody concentration 0.5-1 μg/mL
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CAT-505, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, bovine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells | |
| UniProt accession no. | P04040  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (5 papers) |
| Application: | Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following: • Immunocytochemistry • Immunohistochemistry • Indirect ELISA • Microarray • Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells • Immunofluorescence |
| Biochem/physiol Actions: | Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa. |
| Immunogen: | human erythrocyte catalase |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide |
| Preparation Note: | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |