Caspase 8 human
SIGMA/C1099 - ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine tagged), buffered aqueous solution, ≥1,000 units/mg protein
Synonym: FLICE; MASH; Mch5
MDL Number: MFCD01865996
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| form | buffered aqueous solution |
| mol wt | ~30 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli (C-terminal histidine tagged) |
| shipped in | dry ice |
| specific activity | ≥1,000 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | Q14790  |
| Biochem/physiol Actions: | Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. |
| Biochem/physiol Actions: | Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells. |
| General description: | Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag. |
| Other Notes: | One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 °C. |
| Packaging: | 10 μg in poly bottle |
| Physical form: | Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H319 - H360D |
| Precautionary statements | P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-35-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥1,000 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |