Monoclonal Anti-Caspase 7 antibody produced in rat
SIGMA/C1104 - clone 11E4, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD01633625
Product Type: Chemical
Western Blotting Whole extract of cultured Human acute T cell leukemia Jurkat cells were separated on SDS-PAGE and probed with Monoclonal Anti-Caspase 7 antibody produced in Rat, Clone: 11E4 (Cat. No. C1104). The antibody was developed with 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 2 μg/mL antibody 2. 4 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 11E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen (full length) 35 kDa |
| antigen (subunit) 20 kDa | |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 2-4 μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells | |
| UniProt accession no. | P55210   |
| Application: | Monoclonal Anti-Caspase 7 antibody produced in rat is suitable for the following applications: • Immunoprecipitation • Microarray • Western blotting at a concentration of 2-4μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells. |
| Biochem/physiol Actions: | Caspase-3 and -7 plays a central role in the execution-phase of cell apoptosis. The gene for caspase 7 (also known as Mch3, CMH-1 and ICELAP3) encodes a “short” prodomain protein product of 35 kDa that belongs to the Ced3/ICE family of cysteine proteases. Caspase 7 undergoes acleavage upon activation by caspase-3, -9 and -10, and granzyme B, leading to p20 and p12 subunits. It is an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic Tlymphocyte- induced cell killing in vivo. Monoclonal antibodies reacting specifically with caspase 7 are useful tools for the study of protease networks involved in development and regulation and governing the life and death of cells and tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caspase-7 is a apoptosis-related cysteine peptidase, commonly known as CASP7. It is a human protein encoded by the CASP7 gene. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins and is an executioner protein of apoptosis. |
| Immunogen: | recombinant p20 subunit of human caspase-7. |
| Preparation Note: | The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |