Caspase 3 human
SIGMA/C1224 - ≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein
Synonym: Apopain; CPP32; Yama
MDL Number: MFCD01634593
Product Type: Chemical
Specific Inhibition of Caspase 3 Activity. Caspase 3 (50 ng) activity was assayed in 96 well multiwell plates, using the colorimetric substrate Ac-DEVD-pNA (200 μM) at various concentrations of the following inhibitors: Ac-DEVD-CHO (caspase 3 inhibitor), Ac-YVAD-CHO (caspase 1 inhibitor) and Ac-IETD-CHO (caspase 8 inhibitor). The assay was performed at 25 °C for 35 minutes, in a total volume of 100 μl.
| assay | ≥85% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~30 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli (C-terminal histidine-tagged) |
| shipped in | dry ice |
| specific activity | ≥1.0 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P42574  |
| Application: | Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay. This product may also be used in cell-based apoptosis assay. |
| Biochem/physiol Actions: | Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B. |
| Biochem/physiol Actions: | Member of the CED-3 subfamily of caspases and responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. |
| General description: | Research area: Apoptosis Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag. |
| Other Notes: | One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp- |
| Packaging: | 10 μg in glass bottle |
| Physical form: | Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS |
| Preparation Note: | Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| activity | specific activity: ≥1.0 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.22.56 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |