Anti-Calcium Channel (α1A Subunit) (P/Q-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit
SIGMA/C1353 - affinity isolated antibody, lyophilized powder
Synonym: Anti-APCA; Anti-BI; Anti-CACNL1A4; Anti-CAV2.1; Anti-DEE42; Anti-EA2; Anti-EIEE42; Anti-FHM; Anti-HPCA; Anti-MHP1; Anti-SCA6
MDL Number: MFCD02095950
Product Type: Chemical
Immunohistochemistry Anti-Cav2.1: Cat. No. C1353: Immunohistochemical staining of Mouse cerebellum with Anti-Cav2.1 antibody, (1:100). A. Cav2.1 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.
Immunoblotting
Anti-Cav2.1: Cat. No. C1353: Western blot analysis of Rat brain membranes.
Lanes
1. Anti-Cav2.1antibody (1:200).
2. Anti-Cav2.1 antibody, preincubated with the control peptide antigen.
Anti-Cav2.1: Cat. No. C1353: Western blot analysis of Rat brain membranes.
Lanes
1. Anti-Cav2.1antibody (1:200).
2. Anti-Cav2.1 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| mol wt | antigen (major) 190 kDa |
| antigen (minor) 210 kDa | |
| Quality Level | 100  |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100 |
| western blot: 1:200 | |
| UniProt accession no. | P54282  |
| Application: | Anti-Calcium Channel (α1A Subunit) (P/Q-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit is suitable for immunohistochemistry at a dilution of 1:100 and western blotting at a dilution of 1:200. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) |
| Biochem/physiol Actions: | The Cav2.1 P/Q voltage-dependent calcium channel is encoded by the CACNA1A gene in humans. It is a pore-forming essential subunit embedded in the plasma membrane. Mutation in this gene causes a number of human neurologic diseases including familial hemiplegic migraine. CACNA1A is associated with both hemiplegic migraine and migraine with aura in patients. Mutation in this gene is the cause of Type 2 episodic ataxia (EA2) the most common subtype among a group of rare hereditary syndromes characterized by recurrent attacks of ataxia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 865-881 of the α1A subunit of rat brain voltage-gated calcium channel (VGCC, CNA1) (with additional N-terminal lysine and tyrosine) conjugated to KLH. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |