Monoclonal Anti-Caspase 11 antibody produced in rat
SIGMA/C1354 - clone 17D9, purified from hybridoma cell culture
MDL Number: MFCD02095975
Product Type: Chemical
Immunoblotting Cell and tissue lysates were separated on SDS-PAGE and probed with 8 μg/mL Rat Monoclonal Anti-Caspase 11, Clone: 17D9 (Cat. No. C1354). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. Mouse spleen lysate (LPS stimulated) 2. Rat brain 3. Rat kidney 4. HEK-293T
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 17D9, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 5-10 μg/mL using mouse EL4 cell extracts. |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P70343  |
| Application: | Monoclonal Anti-Caspase 11 antibody can be used in immunohistochemistry and immunoprecipitation. It is also useful in immunoblotting |
| Biochem/physiol Actions: | Caspases are normally produced in the cell as inactive procaspases. Caspase 11 can induce apoptosis in cultured cells due to overexpression. Monoclonal antibodies reacting specifically with caspase 11 are useful tool for the study of the protease network, involved in development and regulation, governing the life and death of cells and tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caspases are proteases having similar structure, substrate specificity and can be divided into three subfamilies based upon their amino acid sequence homology.Monoclonal Anti-Caspase 11 (rat IgG2a isotype) is derived from the 17D9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant p30 subunit of mouse caspase 11. Caspase 11 (also known as Ich-3) gene encodes two protein products of 38 and 43 kDa that belong to the Ced3/ICE family of cystein proteases. Caspase 11 is an “upstream” caspase with a long prodomain. Caspase 11 is identified in murine and shares homology with the human caspase 4 and 5. |
| Immunogen: | recombinant p30 subunit of mouse caspase 11. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |