CDK6/CyclinD1, active, His/GST tagged human
SIGMA/C1374 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CCND1; Cell division protein kinase 6; BCL1; CDK6; D11S287E; MGC59692; PLSTIRE; PRAD1; U21B31
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | CDK6 subunit ~40 kDa |
| cyclin D1 ~61 kDa | |
| product line | PRECISIO® Kinase |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| solubility | soluble |
| water: soluble | |
| specific activity | 7.9-10.7 nmol/min·mg |
| storage temp. | −70°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P24385  |
| Q00534 |
| Application: | CDK6/CyclinD1, active, His/GST tagged human has been used in: In vitro CDK6 activity assay to evaluate the effects of small-molecule inhibitors for therapeutic haematopoietic stem cells (HSC) expansion. |
| Biochem/physiol Actions: | Cyclin Dependent Kinases6 (CDK6) is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. CDK6 kinase activity is detected during the mid-G1 phase of the cell cycle. It plays a crucial role in phosphorylating and regulating the activity of the retinoblastoma (RB), a tumor suppressor protein. Moreover, CDK6 has active involvement during transcription in hematopoietic stem cells (HSC) and tumor cells. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. The activation of cyclin D1-dependent kinase activity is found to be necessary to trigger the process of apoptosis. |
| General description: | Research area: APOPTOSIS This recombinant product was expressed by baculovirus in Sf9 insect cells using an N-terminal His-tag on CDK6, and a GST-tag on Cyclin D1. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Sodium Phosphate, pH 7.0, with 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 7.9-10.7 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |