Cholesterol Esterase from Pseudomonas sp.
SIGMA/C1403 - lyophilized powder, ≥200,000 units/g protein
Synonym: Cholesterol Esterase from Pseudomonas fluorescens; Sterol-ester acylhydrolase
CAS Number: 9026-00-0
EC Number: 232-808-6
MDL Number: MFCD00071052
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form at 5 °C) in K-phosphage buffer, ph 7.0
Figure 3. pH Activity; 37 °C, in 0.2 M K-phosphate buffer
Figure 4. Temperature activity; in 0.1 M K-phosphate buffer, pH 7.0
Figure 5. pH Stability; 25 °C, 24-hr treatment with 0.2 M Britton-Robinson buffer
Figure 6. Thermal stability; 10-min. treatment with 20 mM K-phosphate buffer, pH 7.5 containing 2 mM MgCl2 and 0.5 mM EDTA • NA3
| composition | protein, ≥40% biuret |
| form | lyophilized powder |
| mol wt | ~300 kDa |
| Quality Level | 200  |
| specific activity | ≥200,000 units/g protein |
| storage temp. | −20°C |
| Application: | This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis. |
| Biochem/physiol Actions: | Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis. |
| General description: | Stability: Stable at –20°C for at least one year Isoelectric point: 5.9 ± 0.1 Michaelis constants: 5.4 x 10‾5M (Linoleate), 6.6 x 10‾5M (Oleate) 3.7 x 10‾5M (Linolenate), 1.5 x 10‾4M (Palmitate) 1.2 x 10‾4M (Myristate), 2.3 x 10‾5M (Stearate) Inhibitors: Hg++, Ag+, ionic detergents Optimum pH: 7.0 − 9.0 Optimum temp: 40°C pH Stability: pH 5.0 − 9.0 (25°C, 24hr) Thermal stability: Below 55°C (pH 7.5, 10min) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200,000 units/g protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |