Anti-Calcium Channel (α1B Subunit) (N-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit
SIGMA/C1478 - affinity isolated antibody, lyophilized powder
MDL Number: MFCD02095951
Product Type: Chemical
Immunohistochemistry Anti-Cav2.2: Cat. No. C1478: Immunohistochemical staining of Mouse cerebellum with Anti-Cav2.2 antibody, (1:100). A. Cav2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje cells with Mouse Anti-Calbindin 28K (green) demonstrates the restriction of Cav2.2 to cell bodies but not to dendrites in the molecular layer. C. Merged image of panels A and B.
Immunocytochemistry Anti-Cav2.2: Cat. No. C1478: Immunocytochemical staining of paraformaldehyde-fixed and permeabilized Rat dorsal root ganglion (DRG) primary culture. A. Cells were stained using Anti-Cav2.2 antibody, (1:200) followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.
Immunoblotting Anti-Cav2.2: Cat. No. C1478: Western blot analysis of Rat brain membranes. Lanes 1. Anti-Cav2.2 antibody, (1:200). 2. Anti-Cav2.2 antibody, preincubated with the control antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| mol wt | antigen 210 kDa (low) |
| antigen 240 kDa (high) | |
| Quality Level | 100  |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using rat brain sections |
| immunoprecipitation (IP): suitable | |
| western blot: 1:100-1:200 using rat brain membranes | |
| UniProt accession no. | Q02294  |
| Application: | Anti-Calcium Channel (α1B Subunit) (N-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit is suitable for the following applications: • immunocytochemistry using rat brain sections • immunoprecipitation • western blotting at a dilution of 1:100-1:200 using rat brain membranes |
| Application: | Anti-Calcium Channel (α1B Subunit) (N-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit has been used in immunofluorescence analysis. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) |
| Biochem/physiol Actions: | Calcium channel, voltage-dependent, N type, α1B subunit (CACNA1B) plays a vital role in regulation of trafficking and biophysical properties of voltage-gated calcium channels. Members of voltage-gated calcium channel family act as key transducers of cell surface membrane potential changes to local intracellular calcium transients that initiate various physiological events. The N-type voltage-gated calcium channels (VGCCs) expressed mainly in the neurons, facilitate neurotransmitter release at the sympathetic nerve terminals. CACNA1B is expressed on presynaptic nerve terminals of nociceptors control neurotransmitter release. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The calcium channel, voltage-dependent, N type, α1B subunit (CACNA1B) or Cav2.2 gene consists of 47 exons and 46 introns, spanning 244kb. The gene is mapped to human chromosome 9q34. The encoded protein is a member of the voltage-gated calcium channel family. |
| Immunogen: | synthetic peptide corresponding to amino acids 851-867 of the α1B subunit of rat brain voltage-gated calcium channel (VGCC, CNB1), with additional N-terminal lysine and tyrosine, conjugated to KLH.1 |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |