Cyclohexanone Monooxygenase from Acinetobacter sp.
SIGMA/C1622 - ≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli
CAS Number: 52037-90-8
MDL Number: MFCD00146601
Product Type: Chemical
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| concentration | ≥12 U/mL |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | Cyclohexanone monooxygenase has been used in a study that cloned and overexpressed the 2-oxo-Δ(3)-4,5,5-trimethy |
| Biochem/physiol Actions: | Purified cyclohexanone monooxygenase has a molecular weight of 59,000 Da. It is a versatile oxygenation catalyst. The enzyme uses the bound FAD-4a-OOH oxygenating intermediate to initiate transfer of oxygen to electrophilic substrate sites. The reaction consequently yields the corresponding sulfoxide and selenoxide products. This enzyme is also capable of oxygenating at nitrogen, trivalent phosphorus, and boron sites in boronic acids. Hence, it is one of the most broad-based flavoprotein oxygenases known. |
| Other Notes: | 1 unit corresponds to the amount of enzyme which catalyzes the cyclohexanone-stimulated oxidation of 1 μmol of NADPH per minute at pH 9.0 and 30 °C |
| Physical form: | Suspension in 80% saturated ammonium sulfate, 20 mM K-Na-phosphate buffer pH 7, 3.5 mM 1,4-Dithioerythritol (DTE) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.14.13.22 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |