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Cholera Toxin B subunit

SIGMA/C1655 - FITC conjugate, lyophilized powder

Synonym: CTB

MDL Number: MFCD02356522
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-C1655-250UG 250 µg
 $152.00
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45-C1655-.5MG 0.5 mg
 $262.00
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ELISA ELISA plate coated with Monosialoganglioside GM1 from Bovine brain (Cat. No. G7641) incubated with serially diluted Cholera Toxin B subunit (Cat. No. C1655) and Anti-Cholera Toxin antibody produced in Rabbit (Cat. No. C3062) followed by Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) to determine Cholera Toxin B subunit binding to Monosialoganglioside GM1 by indirect ELISA.

 

composition Protein, ~20% Lowry
conjugate FITC conjugate
extent of labeling ~1.0 mol FITC per mol protein
form lyophilized powder
InChI 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChI key YDXZSNHARVUYNM-UHFFFAOYSA-N
mol wt ~12 kDa
Quality Level 200 
SMILES string CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
storage temp. 2-8°C
Analysis Note: Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Application: Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
• to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
• to stain epididymal sperms of mice
• to stain peritoneal macrophages of mice
Biochem/physiol Actions: The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
Biochem/physiol Actions: The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Features and Benefits: This compound is a featured product for Cyclic Nucleotide research. Click here  to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm .
Physical form: Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Storage Temp. 2-8°C
UNSPSC 12352200

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