Acetylcholinesterase human
SIGMA/C1682 - recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (Lowry)
Synonym: AChE; Acetylcholine acetylhydrolase
CAS Number: 9000-81-1
EC Number: 232-559-3
MDL Number: MFCD00081268
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
The activity of Acetylcholinesterase human recombinant (Cat. No. C1682) was kineticaly measured by monitoring the hydrolysis of acetylthiocholine using Elman′s reagent. The specific activity is greater than 1,000 units/mg.
| form | lyophilized powder |
| mol wt | 64.6 kDa |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| specific activity | ≥1,000 units/mg protein (Lowry) |
| storage temp. | −20°C |
| UniProt accession no. | P22303  |
| Analysis Note: | The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. |
| Application: | Acetylcholinesterase human has been used: • as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-S • to stimulate human fibroblasts • to study its in vitro catalytic activity and to determine the effects of metals, H2O2 and OH radicals on the activity |
| Biochem/physiol Actions: | Acetylcholinesterase (ACh |
| Biochem/physiol Actions: | Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid. |
| General description: | Acetylcholinesterase (ACh |
| Other Notes: | One unit will hydrolyze 1.0 μmole of acetylthiocholine to thiocholine and acetate per minute at pH 8.0 at 37 °C. |
| Packaging: | 0.1 mg in serum bottle |
| Physical form: | This product is supplied as a lyophilized powder. Lyophilized from 0.22 μm filtered solution in 50mM phosphate buffer pH8. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 units/mg protein (Lowry) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.7 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |