Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
SIGMA/C1801 - clone PCK-26, ascites fluid
Synonym: Monoclonal Anti-pan-Cytokeratin
Product Type: Chemical
Immunofluorescence
A mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431) grown on coverslips, fixed and stained with Monoclonal Anti-Pan Cytokeratin, clone no. PCK-26 (Cat. No. C1801) (green); Monoclonal Anti-Vimentin, clone no. VIM-13.2 (Cat. No. V5255) (red); and Hoechst No. 33258 (Cat. No. B2883) (blue) nuclear stain.
From G. MacKenzie, Immunogen Inc., Cambridge, MA.
A mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431) grown on coverslips, fixed and stained with Monoclonal Anti-Pan Cytokeratin, clone no. PCK-26 (Cat. No. C1801) (green); Monoclonal Anti-Vimentin, clone no. VIM-13.2 (Cat. No. V5255) (red); and Hoechst No. 33258 (Cat. No. B2883) (blue) nuclear stain.
From G. MacKenzie, Immunogen Inc., Cambridge, MA.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26 (Cat. No. C1801), followed by Goat Anti Mouse IgG (Fab)-FITC (Cat. No. F5262).
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26 (Cat. No. C1801). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:300 2. Antibody dilution 1:500 3. Antibody dilution 1:1,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PCK-26, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300 |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:300 using protease-idgested, formalin-fixed, paraffin-embedded sections of human or animal tissues | |
| western blot: suitable |
| Application: | Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti-Cytokeratin, pan is a broadly reactive group-type antibody which recognizes an epitope present in most human epithelial tissues. It facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors. Monoclonal Anti-Cytokeratin, pan may be used for the localization of cytokeratins using various immunochemical assays such as immunoblotting, dot blotting, and immunohistochemstry (immunofluorescence and immunoenzymatic staining). A minimum antibody titer of 1:300 was determined by indirect immunofluorescent staining of protease digested, formalin-fixed, paraffin-embedded sections of human or animal tissues. |
| Biochem/physiol Actions: | Pan-cytokeratin along with the expression of CXCR4, may be a prognostic biomarker for non-small cell lung cancer (NSCLC). |
| Biochem/physiol Actions: | The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratin belongs to the member of intermediate filament (IF) protein family. |
| General description: | Cytokeratins, a group comprising at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 1, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and it is modified post-translationally in the terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. |
| Immunogen: | cytokeratin from human epidermis. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |