Monoclonal Anti-Pan Cadherin antibody produced in mouse
SIGMA/C1821 - clone CH-19, ascites fluid
Synonym: Monoclonal Anti-Pan Cadherin
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: The use of automated quantitative analysis to evaluate epithelial-to-mesenchymal transition associated proteins in clear cell renal cell carcinoma. By: O′Mahony, F. C., Faratian, D., et al. in PLoS One, 2012. PubMed ID: 22363672 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0031557), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded chicken heart sections with 1:500 Monoclonal Anti-Pan Cadherin, Clone: CH-19 (Cat. No. C1821) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence MDBK cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 1:500 Monoclonal Anti-Pan Cadherin, Clone: CH-19 (Cat. No. C1821). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Madin Darby Bovine Kidney (MDBK) extract was separated on SDS-PAGE and probed with Monoclonal AB to PAN Cadherin (MO) CL: CH-19 (PREPK) (Cat. No. C1821). The antibody was developed with HRP APA Mouse Fab ads/HIgG conjugate (Cat. No. A9917) and a NBT/BCIP substrate. Antibody dilution 1/2,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | CH-19, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 135 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections |
| indirect immunofluorescence: 1:500 using cultured MDBK cells | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P12830  |
| Application: | Monoclonal Anti-Pan Cadherin antibody produced in mouse has been used in: • fluorescence imaging • immunohistochemistry • western blotting • immunoblotting • immunofluorescence |
| Application: | Monoclonal Anti-Pan Cadherin may be used for: immunochemical and immunocytochemical detection of members of the cadherin family in normal and neoplastic cells and tissues; identification of genetically engineered proteins containing the C-terminal cadherin tail; screening of cDNA expression libraries for identification of novel members of the cadherin family and demonstration of adherens-type cell-cell junctions irrespective of their cadherin subtype. |
| Biochem/physiol Actions: | Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type. |
| Biochem/physiol Actions: | Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin family. The cadherins migrate in the molecular weight range of 125 to 140 kDa and are tissue dependent. The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin′s-fixed and acetone-fixed tissue sections. It reacts with cultured cell line preparations. Cross-reaction has been observed with human, rabbit, bovine, goat, sheep, dog, pig, guinea pig, hamster, cat, rat, mouse, psammomys, chicken, frog and snake. |
| Biochem/physiol Actions: | Neural-cadherin (N-cadherin) has an oncogenic role in bladder cancer, breast cancer, prostate cancer and melanoma. It stimulates the heterotypic adhesion of melanoma cells to dermal fibroblast cells. N-cadherin acts as a critical modulator for the migration of neuronal progenitors. It is essential for the proper cohesion and polarisation of neuro |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+ -dependent cell-cell adhesion. The N-terminal part of these molecule is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and α-actinin. Cadherins that are primarily located in areas of cell-cell contacts are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and in tumor invasiveness and metastasis. Multiple cadherins have been characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific polyclonal antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin has been prepared. |
| General description: | Neural-cadherin (N-cadherin) is a member of the classic cadherin family. It is mainly expressed in neural and mesenchymal tissues. |
| Immunogen: | synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |