Anti-Calcium Channel CaV3.2 (α1H) antibody produced in rabbit
SIGMA/C1868 - affinity isolated antibody, lyophilized powder
Synonym: Anti-CACNA1HB; Anti-Cav3.2; Anti-ECA6; Anti-EIG6; Anti-HALD4
MDL Number: MFCD09265354
Product Type: Chemical
Immunohistochemistry Anti-Cav3.2: Cat. No. C1868: Immunohistochemical staining of Mouse cerebellum frozen sections with Anti-Cav3.2 antibody, (1:100). A. Cav3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with Mouse Anti-Parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of Cav3.2 adjacent to Purkinje cells.
Immunohistochemistry Anti-Cav3.2: Cat. No. C1868: Immunohistochemical staining of Rat dorsal root ganglion (DRG) frozen sections with Anti-Cav3.2 antibody, (1:50). Staining is specific for DRG. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342 is used as the counterstain.
Immunocytochemistry Anti-Cav3.2: Cat. No. C1868: Immunocytochemical staining of paraformaldehyde-fixed and permeabilized Rat dorsal root ganglion (DRG) primary culture. A, D. Immunocytochemical staining using Anti-Cav3.2 antibody (1:200), followed by Goat Anti-Rabbit-AlexaFluor-488 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged image of panels A and B. F. Merged image of panels D and E. Magnification: A-C: ×20; D-F: ×100
Immunoblotting
Anti-Cav3.2: Cat. No. C1868: Western blot analysis of Rat DRG lysates.
Lanes
1. Anti-Cav3.2 antibody, (1:200).
2. Anti-Cav3.2 antibody, preincubated with the control peptide antigen.
Anti-Cav3.2: Cat. No. C1868: Western blot analysis of Rat DRG lysates.
Lanes
1. Anti-Cav3.2 antibody, (1:200).
2. Anti-Cav3.2 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:200 using lysate from the ND7/23 cell line |
| UniProt accession no. | Q9EQ60  |
| Application: | Anti-Calcium Channel CaV3.2 (α1H) antibody produced in rabbit is suitable for western blotting at a dilution of 1:200 using lysate from the ND7/23 cell line. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Calcium channel, voltage-dependent, T type, α1H subunit (CACNA1H) plays an essential role in regulating neuronal excitability and network oscillations in the brain. Mutations in the gene are associated with various forms of idiopathic generalized epilepsy. Gaining-of-function mutation in CACNA1H increases seizure susceptibility by directly altering neuronal electrical properties and indirectly by changing gene expression. CACNA1H acts as an important mediator of Ca2+ entry near the resting membrane potential. It plays a pivotal role in processing of pain signals and may serve as a novel target for development of drugs for the treatment of intractable pain resistant to currently available analgesics. CACNA1H up-regulation is involved in the pathophysiology of inflammatory, neuropathic and visceral pain. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calcium channel, voltage-dependent, T type, α1H subunit (CACNA1H) is a protein encoded by the CACNA1H gene in humans and is mapped to chromosome 16p13.3. The proteins are membrane protein machinery performing selective permeation of external calcium ions. The α1H channel is sensitive to mibefradil, a nondihydropyridine Ca2+ channel blocker. CACNA1H is abundantly expressed in both peripheral and central endings of the primary afferent neurons, regulating neuronal excitability and release of excitatory neurotransmitters. |
| Immunogen: | synthetic peptide CHVEGPQERARVAHS corresponding to amino acid residues 581-595 of rat CaV3.2. Epitope location: intracellular loop connecting D1-D2. Mouse, bovine, and canine have 14/15 residues identical; human has 13/15 residues identical. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |