Calcineurin from bovine brain
SIGMA/C1907 - lyophilized powder, ≥2,500 units/mg protein
Synonym: Calcium/
CAS Number: 90371-51-0
MDL Number: MFCD00130697
Product Type: Chemical
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| biological source | bovine |
| composition | Protein, 0.3-1.7% Lowry |
| form | lyophilized powder |
| mol wt | dimer ~77 kDa |
| subunit mol wt 19-58 kDa | |
| Quality Level | 200  |
| solubility | H2O: soluble |
| specific activity | ≥2,500 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P48452  |
| Analysis Note: | Sigma tests activity in 80 mM Tris, pH 7.5 with 65 mM KCl, 8 mM MgSO4 and 0.3% albumin. |
| Application: | Calcineurin from bovine brain has been used: • as a positive control in western blot analysis of oocytes and cumulus cells proteome • as a positive control in calmodulin (CaM)-agarose binding assay • to test its phosphatase activity in the presence of okadaic acid |
| Biochem/physiol Actions: | Calcineurin (CaN) activity is stimulated by nickel (Ni2+) and manganese (Mn2+) ions. It participates in the coupling of Ca2+ signals. CaN may regulate oocyte growth and meiotic maturation in porcine. It also participates in gene regulation, cell survival, and death. |
| Biochem/physiol Actions: | Calcineurin is a cyclosporin-sensitive, calcium-regulated, serine-threonine protein phosphatase with broad substrate specificity. It is the major calmodulin-binding protein found in the brain. First identified as an inhibitor of the calmodulin activation of phosphodiesterase 3′:5′ cyclic nucleotide (PDE), calcineurin has similar effects on adenylate cyclase. Serves as a key enzyme involved in T-cell activation. Also involved in the hyperphosphorylation of tau protein in Alzheimer′s disease and has been shown to prevent calpain-mediated proteolysis of tau in differentiated PC12 cells. |
| Biochem/physiol Actions: | The major calmodulin-binding protein found in the brain. A key enzyme involved in T-cell activation and hyperphosphorylation of tau protein in Alzheimer′s disease. |
| General description: | Calcineurin (CaN) comprises CaN A and CaN B subunits. It exists as a heterodimer with a calmodulin-binding domain, catalytic site, a CaN B binding domain, and an autoinhibitory domain. |
| Other Notes: | One unit will cause a 50% inhibition of the activated phosphodiesterase, 3′:5′-cyclic nucleotide (P 9529) activity when assayed with two units of activator (P 2277) and 0.1 mM Ca2+ in an enzyme coupled system at pH 7.5 at 30 °C. |
| Physical form: | Lyophilized powder containing 0.5% EGTA, buffer salts and stabilizers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,500 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |